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KMID : 0370220080520030172
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Yakhak Hoeji
2008 Volume.52 No. 3 p.172 ~ p.175
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Planting Condition of Korean Cannabis Derived from Stable Isotype Ratio & Tetrahydrocannabiol Contents
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Lee Jae-Sin
Park Yong-Hoon
Lee Jong-Sook
Jeong Jin-Il
Lim Mi-Ae
Chung Hee-Sun
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Abstract
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Stable isotope ratio of carbon and nitrogen (δ13C & δ15N), and ? 9-tetrahydrocannabinol (THC) contents were measured on 37 Korean cannabis and 10 commercial grade marijuana seized in Korea. Factors influencing on the measured values and their variations were investigated. δ13 value of cannabis is specified mainly by water availability. Korean cannabis showed relatively low δ13 values ranging -33.29~-27.01‰ (mean=-31.01‰), which reflect geographic conditions of Korea where is rainy, especially during summer. δ15N values, which reflect individual planting conditions, were relatively high up to -0.5~18.0‰ (mean=6.44‰). It reflects characteristics of Korean cannabis growing wild in forest or cultivated in fertile soil. Tetrahydrocannabinol is the major hallucinogenic compound of cannabis. Ethanol extracts of cannabis leaves were derivatized by N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), and the derivatives were analyzed by GC-MS in selected ion monitoring (SIM) mode. THC contents of Korean cannabis ranged 0.11~4.34% (mean=1.47%), which were relatively low compared with commercial grade marijuana.
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